The objectives of Introducing Medical Diagnostics Course in Schools is to help students understand the organization of Hospitals, Research labs, Diagnostic labs and to equip students with the skills needed to work in a hospital or a diagnostics lab.

Part B: Vocational Skills (50 Marks)

Unit 1: Investigation Urine & Faces Analysis

  • Introduction.
  • Maintenance & Equipments of Pathology Lab.
  • Preparation of Reagents.
  • Urine.
  • Formation and composition.
  • Collection Preservation Gravity & PH.
  • Examination – Physical.
  • Examination – Chemical.
  • Sugar.
  • Ketone Bodies, Bile.
  • Blood, Crystals.
  • Parasites & Abnormal Cells.
  • Feces – Formation, Physical & Chemical Examination.
  • Preparation of stool sample for microscopic examination.
  • Sputum Examination.
  • Assessment.

Unit 2: Body Fluids

  • Body Fluids.
  • Cerebro spinal fluid. Synovial fluid and Pleural fluid Pericardial fluids Peritoneal fluids.
  • Other fluids collected stransudateor exudates.
  • Semen Analysis – Collection, Physical & Chemical examination.
  • Sperm count – Microscopic examination & Motility.
  • Assessment.

Unit 3: Process & Investigations

  • Reagents – Preparation and their uses.
  • Personnel care and protection Disposal of Bio-Medical waste Smear Making.
  • Staining Methods.
  • Osmotic fragility test.
  • Differential Counts.
  • Cellcounts – RBC, WBC, and Platelets Eosinophil & Reticulocyte count ESR.
  • LE Cell.
  • Haemopoeisis – Erythrocytes Hemoglobin – Estimation Packed Cell Volume, Indices Hematocrit and Red cellindices Anemia.
  • Leukocytes.
  • Coagulation Factors.
  • Coagulation disorders – Bleeding & Clotting Time.
  • Bone marrow study.
  • Assessment.

Unit 4: Blood Bank & Transfusion

  • Blood Bank.
  • Material & equipment Reagents – preparation Protocols.
  • Storage & Preservation.
  • Records in Blood bank.
  • ABO System.
  • Subgroups in ABO System.
  • Practical importance of Other blood groups.
  • Rh System.
  • Antibody titers.
  • Blood grouping techniques Problems in blood grouping Donor Motivation.
  • Donors election & Registration.
  • Blood Collection.
  • Storage, Preservation & Processing of blood.
  • Quality control.
  • Investigation of transfusion, reactions.
  • Grouping & cross matching Direct and Indirect Coombs test Mandatory Test.
  • Assessment.

Unit 5: Lab Process

  • Materials, Equipment & Techniques.
  • Biopsy, Autopsy.
  • Collection, Preservation & Labeling of Slides, Blocks, Specimens.
  • Techniques.
  • Grossing Methods.
  • Fixatives.
  • Processing of the tissues including Bone.
  • Embedding Section Cutting Staining & Mounting Special Stains.
  • Preservation of reports & records.
  • Assessment.

Unit 6: Cytology

  • Techniques Equipment & Procedures – FNAC, Imprintssmear.
  • Vaginal & Buccalsmear, Swabs.
  • Staining procedure and Mounting.
  • Preparation of fluids for Cytological Examination.
  • Immunohisto chemistry.
  • Assessment.