Time: 3 Hours; Marks: 60

Unit–1: Investigation Urine & Faces Analysis

  • Introduction.
  • Maintenance & Equipments of Pathology Lab.
  • Preparation of Reagents.
  • Urine.
  • Formation and composition.
  • Collection Preservation Gravity & PH.
  • Examination–Physical.
  • Examination– Chemical.
  • Sugar.
  • Ketone Bodies, Bile.
  • Blood, Crystals.
  • Parasites & Abnormal Cells.
  • Feces–Formation, Physical & Chemical Examination.
  • Preparation of stool sample for microscopic examination.
  • Sputum Examination.
  • Assessment.

Unit–2: Body Fluids

  • Body Fluids.
  • Cerebro spinal fluid. Synovial fluid and Pleural fluid Pericardial fluids Peritoneal fluids.
  • Other fluids collected stransudateor exudates.
  • Semen Analysis–Collection, Physical & Chemical examination.
  • Sperm count – Microscopic examination & Motility.
  • Assessment.

Unit–3: Process & Investigations

  • Reagents – Preparation and their uses.
  • Personnel care and protection Disposal of Bio-Medical waste Smear Making.
  • Staining Methods.
  • Osmotic fragility test.
  • Differential Counts.
  • Cellcounts – RBC, WBC, and Platelets Eosinophil & Reticulocyte count ESR.
  • LE Cell.
  • Haemopoeisis – Erythrocytes Hemoglobin – Estimation Packed Cell Volume, Indices Hematocrit and Red cellindices Anemia.
  • Leukocytes.
  • Coagulation Factors.
  • Coagulation disorders – Bleeding & Clotting Time.
  • Bone marrow study.
  • Assessment.

Unit–4: Blood Bank & Transfusion

  • Blood Bank.
  • Material & equipment Reagents – preparation Protocols.
  • Storage & Preservation.
  • Records in Blood bank.
  • ABO System.
  • Subgroups in ABO System.
  • Practical importance of Other blood groups.
  • Rh System.
  • Antibody titers.
  • Blood grouping techniques Problems in blood grouping Donor Motivation.
  • Donors election & Registration.
  • Blood Collection.
  • Storage, Preservation & Processing of blood.
  • Quality control.
  • Investigation of transfusion, reactions.
  • Grouping & cross matching Direct and Indirect Coombs test Mandatory Test.
  • Assessment.

Unit–5: Lab Process

  • Materials, Equipment & Techniques.
  • Biopsy, Autopsy.
  • Collection, Preservation & Labeling of Slides, Blocks, Specimens.
  • Techniques.
  • Grossing Methods.
  • Fixatives.
  • Processing of the tissues including Bone.
  • Embedding Section Cutting Staining & Mounting Special Stains.
  • Preservation of reports & records.
  • Assessment.

Unit–6: Cytology

  • Techniques Equipment & Procedures – FNAC, Imprintssmear.
  • Vaginal & Buccalsmear, Swabs.
  • Staining procedure and Mounting.
  • Preparation of fluids for Cytological Examination.
  • Immuno histo chemistry.
  • Assessment.