Time: 3 Hours; Marks: 60

Unit–1: Concepts Instruments & Procedures

  • Introduction - scope of biochemistry and clinical biochemistry objectives and scheme of clinical biochemistry teaching.
  • Basic Biochemistry - over view of biomolecules, biochemical transformations. Biochemical organization of cell, tissues, organs and human organism.
  • Ethics and Discipline - Laboratory ethics and discipline. Patient management, Reception, Registration, Biochemical parameters investigations, protocols, documentation.
  • Hazards and safety - physical, chemical and biological hazards, self & patient & equipment safety. Disposal of laboratory waste and the hazardous material.
  • First Aid Measures.
  • Instruments, Principles & Procedures: Basis, uses, parts, installation, glass ware & plastic ware – colorimeters, balances, centrifuges, refrigerators, hot air ovens, water baths, thermometers, vortex, mixers, magnetics stirrers, UV lamp.
  • Cleaning & Maintenance of Equipment.
  • Assessment.

Unit–2: Investigations Separation Procedures & Analysis

  • Separation techniques: basic principles, different types, general techniques and clinical applications of different types of electrophoresis and chromatography.
  • Automation: Basic Principles, different components and general principles of usage and applications.
  • Calcium and Phosphorus: Outlines of mineral metabolism. Principles of estimation of serum calcium and inorganic phosphate and their clinical importance.
  • Urine Proteins: requirements of quantisation of proteins in the urine. Different samples used Principles of samples collection, preservation and analysis.
  • Lipoproteins: Principles of estimation of different lipo protein fractions of lipase, & LDH.
  • Acid–Base balance.
  • Immuno as says.
  • Sample identification and labeling.
  • Types and mechanisms of actions of various anti coagulants and preservatives used Principles of Spectrophotometry and Turbidoemetry.
  • Quantitative analysis.
  • Calorimetery- applications in clinical biochemistry.
  • Units of measurement.
  • Assessment.

Unit–3: Functional Test & Profile 

  • Glucose tolerance test: Concept of tolerance tests. Definition, patient preparation, performance, reporting and interpretation of GTT.
  • Liver functions tests: bilirubin, total proteins, albumin and prothrombin time, turbidity tests and serum enzyme estimations (SGPT, ALPA and GT).
  • Kidney functions tests: Concept of clearance tests, Use of serum NPN substances creatinine clearance, concentration and dilution tests and urine examination.
  • Gastric function tests: Principles of analysis of gastric juice, Concept of basalandmaximal acid outputs, Principles of stimulation tests & tubeless gastric analysis.
  • Thyroid function tests: Principles underlying estimations of various thyroid hormones and their interpretations.
  • Profiles: Concepts of Profile testing.
  • Cardiac Profiles.
  • Lipid Profile.
  • Assessment.

Unit–4: Fundamentals of Microbiology

  • Personal Care.
  • Infection Control.
  • Sterilization Techniques - Autoclave, Hotair oven, Tyndallization & Pasteurization.
  • Equipment – Handling & Maintenance.
  • Assessment.

Unit–5: Bacteriology

  • Bacteriology.
  • Applied Anatomy and Physiology of the Bacterial Cell.
  • Pathogenic organisms.
  • Identification & Isolation of Staphylococcus, Streptococcus, Pneumococcus, Gonococcus, eningococcus, C. diptheria, Mycobacterium tuberculae and M. Leprae, Clostridia, E. Coli, Klebseilla, Salmonella, Shigella, Proteus, Vibrio, Pseudomonas.
  • Anthrax, Plague, Dengue, Japanese encephalitis.
  • Assessment.

Unit–6: Mycology Immunology & Serology Parasitology Virology

  • Introduction & Classification.
  • Enumeration of pathogenic & opportunistic fungi.
  • Introduction.
  • Antigens, Antibodies & Reactions.
  • Hypersensitivity.
  • Introduction.
  • Pathogenic parasites in blood, stool and urine.
  • Viruses – Classification, Cultivation & Enumeration. 
  • Study of the laboratory animals – Sheep, Rabbit, Mice & Guinea Pig.
  • Assessment.