Time: 3 Hours; Marks: 60
Unit–1: Concepts Instruments & Procedures
- Introduction - scope of biochemistry and clinical biochemistry objectives and scheme of clinical biochemistry teaching.
- Basic Biochemistry - over view of biomolecules, biochemical transformations. Biochemical organization of cell, tissues, organs and human organism.
- Ethics and Discipline - Laboratory ethics and discipline. Patient management, Reception, Registration, Biochemical parameters investigations, protocols, documentation.
- Hazards and safety - physical, chemical and biological hazards, self & patient & equipment safety. Disposal of laboratory waste and the hazardous material.
- First Aid Measures.
- Instruments, Principles & Procedures: Basis, uses, parts, installation, glass ware & plastic ware – colorimeters, balances, centrifuges, refrigerators, hot air ovens, water baths, thermometers, vortex, mixers, magnetics stirrers, UV lamp.
- Cleaning & Maintenance of Equipment.
Unit–2: Investigations Separation Procedures & Analysis
- Separation techniques: basic principles, different types, general techniques and clinical applications of different types of electrophoresis and chromatography.
- Automation: Basic Principles, different components and general principles of usage and applications.
- Calcium and Phosphorus: Outlines of mineral metabolism. Principles of estimation of serum calcium and inorganic phosphate and their clinical importance.
- Urine Proteins: requirements of quantisation of proteins in the urine. Different samples used Principles of samples collection, preservation and analysis.
- Lipoproteins: Principles of estimation of different lipo protein fractions of lipase, & LDH.
- Acid–Base balance.
- Immuno as says.
- Sample identification and labeling.
- Types and mechanisms of actions of various anti coagulants and preservatives used Principles of Spectrophotometry and Turbidoemetry.
- Quantitative analysis.
- Calorimetery- applications in clinical biochemistry.
- Units of measurement.
Unit–3: Functional Test & Profile
- Glucose tolerance test: Concept of tolerance tests. Definition, patient preparation, performance, reporting and interpretation of GTT.
- Liver functions tests: bilirubin, total proteins, albumin and prothrombin time, turbidity tests and serum enzyme estimations (SGPT, ALPA and GT).
- Kidney functions tests: Concept of clearance tests, Use of serum NPN substances creatinine clearance, concentration and dilution tests and urine examination.
- Gastric function tests: Principles of analysis of gastric juice, Concept of basalandmaximal acid outputs, Principles of stimulation tests & tubeless gastric analysis.
- Thyroid function tests: Principles underlying estimations of various thyroid hormones and their interpretations.
- Profiles: Concepts of Profile testing.
- Cardiac Profiles.
- Lipid Profile.
Unit–4: Fundamentals of Microbiology
- Personal Care.
- Infection Control.
- Sterilization Techniques - Autoclave, Hotair oven, Tyndallization & Pasteurization.
- Equipment – Handling & Maintenance.
- Applied Anatomy and Physiology of the Bacterial Cell.
- Pathogenic organisms.
- Identification & Isolation of Staphylococcus, Streptococcus, Pneumococcus, Gonococcus, eningococcus, C. diptheria, Mycobacterium tuberculae and M. Leprae, Clostridia, E. Coli, Klebseilla, Salmonella, Shigella, Proteus, Vibrio, Pseudomonas.
- Anthrax, Plague, Dengue, Japanese encephalitis.
Unit–6: Mycology Immunology & Serology Parasitology Virology
- Introduction & Classification.
- Enumeration of pathogenic & opportunistic fungi.
- Antigens, Antibodies & Reactions.
- Pathogenic parasites in blood, stool and urine.
- Viruses – Classification, Cultivation & Enumeration.
- Study of the laboratory animals – Sheep, Rabbit, Mice & Guinea Pig.