Biotechnology Class 12 Syllabus

The syllabus consists of two units: (v) Protein and Gene Manipulation (vi) Cell Culture and Genetic Manipulation. Theory paper is of 70 marks and 3 hours.

Exam Structure

Units Topics Marks
Unit V Protein and Gene Manipulation 40
Unit VI Cell Culture and Genetic Manipulation 30
  Total 70

Unit V: Protein and Gene Manipulation

Chapter 1: Recombinant DNA Technology

Introduction, Tool of Recombinant DNA technology, Making rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.

Chapter 2: Protein Structure and Engineering

Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)

Chapter 3: Genomics and Bioinformatics

Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, Analysis using bioinformatics tools.

Unit VI: Cell Culture and Genetic Manipulation

Chapter 1: Microbial Cell Culture and its Applications

Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.

Chapter 2: Plant Cell Culture and Applications

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Transgenic plants with beneficial traits, Biosafety of transgenic plants

Chapter 3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.