Biotechnology Class 12 Syllabus

The syllabus consists of two units: (v) Protein and Gene Manipulation (vi) Cell Culture and Genetic Manipulation. Theory paper is of 70 marks and 3 hours.

Exam Structure

Units Topics Marks
Unit V Protein and Gene Manipulation 40
Unit VI Cell Culture and Genetic Manipulation 30
  Total 70

Unit V: Protein and Gene Manipulation

Chapter 1: Recombinant DNA Technology

Introduction, Tool of DNA technology, Making DNA, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), Hybridization techniques, DNA library, DNA Sequencing, Site-directed Mutagenesis

Chapter 2: Protein Structure and Engineering

Introduction to the world of proteins, 3-D shape of proteins, Structure-function Relationship in proteins, Purification of proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)

Chapter 3: Genomics and Bioinformatics

Introduction, Genome, Sequencing projects, Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, History of bioinformatics, Sequences and nomenclature, Information sources, Analysis using bioinformatics tools

Unit VI: Cell Culture and Genetic Manipulation

Chapter 1: Microbial Cell Culture and its Applications

Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Scale-up of microbial process, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology, Biosafety issues in microbial technology

Chapter 2: Plant Cell Culture and Applications

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Gene transfer Methods in plants, Transgenic plants with beneficial traits, Biosafety of transgenic plants

Chapter 3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Characterization of cell lines, Methods of gene delivery into cells, Scale-up of animal culture process, Applications of animal cell culture, Stem cell technology, Tissue engineering